Table 2. Variants identified in Ion AmpliSeq Comprehensive Cancer Panel Genes.

Patient	Tumor Type	Gene	Chr	Location	Reference	Variant	Effect	AmpliSeq	ExomeSeq
UH1	P	PAX3	2	223084928	G	A	S SNV		x
	P	PIK3CG	7	106508092	C	T	NS SNV		x
	P	PTCH1	9	98209634	G	A	NS SNV	x	x
	P	TNK2	3	195595319	T	G	NS SNV	x
UH2	P	LLP	3	188584016	C	T	NS SNV		x
	P	LLP	3	188584017	C	T	S SNV		x
	P	BRIP1	17	59763203	C	T	NS SNV		x
	P	DYPD	1	98039494	A	G	S SNV	x
	P	MCL1	1	150551538	C	T	NS SNV	x
	R	CDH5	16	66420931	G	A	NS SNV		x
UH3	P	FLT4	5	180048765	G	A	S SNV		x
	P	PIK3CG	7	106509875	T	A	NS SNV		x
	P	PTEN	10	89653783	T	G	Stopgain		x
	P	TP53	17	7578475	G	A	NS SNV		x
	R	PTEN	10	89653783	T	G	Stopgain		x
	R	TP53	17	7578475	G	A	NS SNV		x
	R	NF1	17	29585518	A	G	NS SNV	x	x

Summary of all variants identified in this study, the majority of which were found in primary tumors. R, recurrent; Chr, chromosome; NS, nonsynonymous; P, primary; SNV, single nucleotide variant.