TABLE 1.

Comparison of Isolation Variables Between Both Methods

	Initial Method (M1)	Modified Method (M2)	P
	n = 7	n = 7
Collagenase concentration, CDUs/mL	2550	3800	—
Collagenase injected, mL/g	0.96 ± 0.02	1.0 ± 0.01	0.102
Net collagenase activity, CDUs/g	3100.2 ± 354.7	4729.2 ± 423.5	0.012
Pancreas distension, %	83.6 ± 6.4	93.6 ± 5.6	0.265
Pancreas weight, g	5.4 ± 0.4	4.5 ± 0.3	0.082
Shaking speed, cycles/min	88	300	—
Time of digestion, min	44.4 ± 0.8	29.4 ± 0.5	<0.001
Time of teasing, min	4.0 ± 0.4	2.4 ± 0.3	0.003
Gradient density, g/mL*	1.119/1.083/1.008 and 1.119/1.077/1.008	1.119/1.077/1.008	—
Purification temperature, °C	22	4	—

^*See text for further details.