Figure 3. Blockade of Fas enhances IFN-γ production by IκBαΔN-Tg T cells in vitro.
WT and IκBαΔN-Tg splenocytes were stimulated in the presence of soluble anti-CD3 mAb (1 µg/ml), in the presence or absence of isotype control or anti-FasL mAb (10 µg/ml). Supernatants were collected at 5 days and assayed for concentration of IFN-γ by ELISA. **p<0.01; ***p<0.001.