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. 2015 May 7;11(5):e1005200. doi: 10.1371/journal.pgen.1005200

Fig 6. PARP2 activity is regulated by PARP1 in response to DNA alkylating agent mitomycin C.

Fig 6

Arabidopsis plants, including (A) parp1-1 or (B) parp1-2 knockout alleles of PARP1, were grown on MS plates supplemented with 0, 20 and 40 μM of mitomycin C (MMC) for two weeks. Total proteins were extracted, separated by SDS-PAGE and analyzed by immunoblotting with anti-PAR antibody. Equivalent loading of lanes was verified using Ponceau S stain. Upper and middle panels of (B) are same blot, showing immunoblot signal after longer and shorter exposure times respectively. Similar results obtained in two separate experiments.