Fig 3. The effect of glibenclamide on channel activity at +20, 0 and -20 mV.
Representative unitary current recordings in control conditions (200/50 mMKCl; cis/trans) and after cis addition of 10, 25, 50 (A) or 100 μM glibenclamide (B) at different voltages. Histogram bars show current amplitudes and Po of reconstituted channels in absence or presence of glibenclamide. The addition of 50 μM glibenclamide resulted in a decreased current jump amplitude and a reduction in Po. 100 μM glibenclamide caused a total channel inhibition at +20 and 0 mV (n = 5), but was ineffective at -20 mV. The significant difference in the channel amplitude and Po values are marked by asterisks (*P < 0.05 and ** Po < 0.01, respectively, n = 5). Data are means ± S.E. Closed levels are indicated by –.