Fig 6. Western blot analysis of Kir6.2 and SURs in the rat microsome fractions.
(A) The goat anti-Kir6.2 antibody led to the identification of a prominent ~55 kDa band in microsome fractions, a molecular weight compatible with a Kir6.2 KATP subunit. (B) ~150 kDa band was detected using the goat anti-SUR2A antibody, whereas the goat anti-human SUR2B and SUR1 antibodies led to the labeling of two bands of 150 and 100 kDa respectively. The 100 kDa may be resulting from proteolysis of the 150 kDa paptide. (C) Western blot indicating that of the Maxi-KCa channel α-subunit (138 kDa) is expressed in rat endoplasmic reticulum membranes.