Table 3. Effects of A, B and F on CaMdr1p overexpressing S. cerevisiae strain AD/CaMDR1A.
| Compound | Solubility in DMSO (mM) | Antifungal activity a | CaMdr1p chemo-sensitization b (nmol/disk) | MICF/C (μM) | FICF | MICC/F (μM) | FICC | FICI |
|---|---|---|---|---|---|---|---|---|
| A | >20 | -/- | 3.2/1.6 | 10 | 0.03 | 0.63 | <0.02 c | <0.05 d |
| B | ~10 | -/7 | 0.8/0.4 | 10 | 0.03 | 1.3 | <0.03 c | <0.06 d |
| F | >20 | 20/14 | ND | ND | ND | ND | ND | ND |
a Antifungal activity expressed as the diameters (mm) of inhibition zones in disk diffusion assays for SD/SD pH 6.8. “-” sign: no activity detected as the inhibition zone diameter was ≤ 6 mm (size of the disk).
b Amount of compound required for detectable chemosensitization in SD/SD pH 6.8 containing 25 μM FLC (1/8th AD/CaMDR1A MIC of FLC).
c Growth inhibition not detected at highest concentration of compound used.
d Synergistic effect
ND: not determined