Skip to main content
. 2015 May 7;11(5):e1005206. doi: 10.1371/journal.pgen.1005206

Fig 5. Integrated estimates of mRNA and protein levels using a structured covariance model (SCM).

Fig 5

A, Integrated estimates of mean steady-state protein and mRNA levels across 58 global measurements reveal a strong genome-wide dependence between (r = 0.93). Estimates are produced for any gene with a detected mRNA (gray marginal densities), and other densities characterize subsets by mRNA and protein detection. B, A single sample from the SCM estimates provides a representative view of mRNA and protein levels. Colors and marginal densities are the same as in A. C, Absolute mRNA level estimates versus single-molecule fluorescence in situ hybridization counts [53]. D, Absolute protein level estimates versus stable-isotope-standardized single reaction monitoring measurements [57]. Dotted lines in B and C show perfect agreement. E, Evidence for active translation of undetected proteins inferred from ribosome profiling. Dashed line shows ranged major-axis regression best fit. Marginal densities show ribosome density (median across five studies, see Methods) for all detected mRNAs (light gray), all mRNAs with a detected mRNA and protein (dark gray), and transcripts with no detected protein (blue).