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. Author manuscript; available in PMC: 2015 May 7.
Published in final edited form as: Methods Mol Biol. 2014;1210:229–237. doi: 10.1007/978-1-4939-1435-7_17

Fig. 1.

Fig. 1

Illustration of pancreas explants on cell culture inserts. (a) Example of a dissected E11.5 pancreas. The stomach and duodenum are still attached to the pancreas. Note the red dashes outlining the dorsal pancreas for illustrative purposes. (b) Nuclepore Membrane inserts floating on top of the liquid medium. For comparison purposes, the well in the upper left corner has no insert. (b′) Zoomed-in image of right lower corner, showing that filter is floating. Note membrane orientation (reflective side up). (c) Explant tissue, shown growing at air/medium interface. Stomach and duodenum are still attached to pancreas, for illustrative purposes. (d) After 3-day culturing, the dissected E11.5 pancreas explants expand, and develop branching structures. St stomach, Pan pancreas, Duo duodenum. (Color figure online)