Table 1. qRT-PCR primer sequences formaize (Zea mays L.) miRNAs, target genes and tubulin.
Primer | Primer sequence (5′-3′) | Primer | Primer sequence (5′-3′) |
---|---|---|---|
Tubulin (F) | GCAGAACAAGAACTCGTCCTACT | Tubulin (R) | TCAGCCTCAGTGAACTCCATCT |
GRF(F) | CAGGGAACTGGTGAACAAACA | GRF(R) | ATCAGTGAATCCGATGAAACG |
SPL(F) | TCGCACCTTCGCTTTACG | SPL(R) | ACTGGCCGCCTCATCACTC |
LAC 17(F) | CAAACCGATACTCCCTGCTCTA | LAC 17(R) | GACCTTGGCATGTCTGGTTATT |
zma-miR156 | TGACAGAAGAGAGTGAGCAC | zma-miRt4 | TGAAGAGAATTGAGGGGGCTA |
zma-miR162 | TCGATAAACCTCTGCATCCA | zma-miRt13 | TCTAGAATCAAAGGCTAATCT |
zma-miR172 | AGAATCTTGATGATGCTGCA | zma-miRt15 | ATGCAGAACAATTTACAGACG |
zma-miR393 | TCCAAAGGGATCGCATTGATCT | zma-miRt17 | TGGTCTTGCCACCGTGTCGTCC |
zma-miR396 | TTCCACAGCTTTCTTGAACTG | zma-miRt21 | GGATGTCGATATTGGAGGGCA |
zma-miR397 | TCATTGAGCGCAGCGTTGATG | zma-miRt28 | TTTGGGGTGGATACGTGGTCA |
zma-miR408 | CTGCACTGCCTCTTCCCTGGC |