Fig 2. Effect of co-culture on inducing macrophages toward the M2 phenotype.
U937 (1×105 cells per well of 24 well plates) was stimulated with LPS (10ng/ml) for 24h after undergoing co-culture with eutopic ESCs for 72h, followed by an analysis of the secretion of IL-10 (A) and IL-12 (B) by ELISA. Surface expression of HLA-DR and CD86 in U937 cells was determined by flow cytometry (C). Eutopic ESCs = ESCs from eutopic endometrium with endometriosis. Data are expressed as mean±SD of independent experiment, performed in triplicate wells with three different samples. *P<0.05 compared with the U937 control, **P<0.01 compared with the U937 control.