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. 2015 May 7;10(5):e0123340. doi: 10.1371/journal.pone.0123340

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© 2015 Anguille et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 3 — Unstimulated NK cells ((-), white bars) and NK cells exposed for 48 hr to IL-15 DCs (grey bars) or IL-4 DCs (dashed bar) were analyzed by flow cytometry for cytotoxicity against K562 and Daudi tumor cells. Target cell killing was determined by Annexin-V/PI staining after 4 hr incubation at an E:T ratio of 5:1. Results are expressed as mean (± SEM) percentage killing, which was calculated using the formula specified in “Methods”. Data are from two independent experiments involving 10 different donors (***, P<0.001; ns, not significant).