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. 2015 May 7;10(5):e0123340. doi: 10.1371/journal.pone.0123340

Table 1. Percentage increase in cell surface marker positivity compared with baseline after 48 hr stimulation of NK cells with either IL-15 DCs or IL-4 DCs.

CD56+ NK cells CD56bright NK cells CD56dim NK cells
IL-15 DCs IL-4 DCs IL-15 DCs IL-4 DCs IL-15 DCs IL-4 DCs
CD56 42.8±9.9% 20.1±9.5%* 51.7±14.3% 26.2±14.9%* 42.2±12.3% 19.8±10.3%*
CD69 33.6±11.1% 13.4±12.3%* 47.6±15.4% 15.3±7.8%* 32.6±11.0% 13.6±12.8%*
CD25 11.0±5.2% 4.5±3.8%* 16.8±10.5% 5.3±5.5%* 9.8±5.4% 4.3±3.6%*
HLA-DR 7.9±3.1% 3.7±1.3%* 7.9±6.0% 2.3±2.7%* 7.9±3.0% 3.9±1.4%*
NKG2D 25.7±15.2% 2.0±5.0%* 38.2±14.2% 11.4±8.6%* 24.1±14.4% 1.8±4.5%*
NKp30 13.3±8.4% 0.5±0.3%* 42.0±12.4% 10.5±9.5%* 9.8±8.5% 0.3±0.2%*
NKp46 11.1±6.3% 0.1±0.2%* 16.2±12.1% 3.1±5.4%* 10.3±6.4% 0.1±0.2%*

Pairwise comparison of the expression of CD56, CD69, CD25, HLA-DR, NKG2D, NKp30 and NKp46 on NK cells (total CD56+ NK cell population, CD56bright subset and CD56dim subset) after 48-hr incubation with IL-15 DCs or IL-4 DCs. Results are presented as mean (± SD) percentage increase in positivity of the indicated cell surface marker as compared with the baseline expression on unstimulated NK cells (calculated using the Overton histogram subtraction algorithm by subtracting the unstimulated NK cell control histogram from the DC-stimulated NK cell sample histogram). Asterisks indicate a statistically significant difference in cell surface marker expression between IL 15 DC-stimulated and IL-4 DC-stimulated NK cells (n = 6; *, P<0.05).