Fig 2. pATM and p53BP1 are induced in the presence or absence of replication compartments during EBV lytic cycle reactivation.

2089 cells (A, C) or ΔG4ΔG5 cells (B, C) were transfected with an empty vector (CMV) or a plasmid bearing the wild-type ZEBRA gene. Cells were fixed and double-stained for Rta and pATM (A, B). Rta-positive cells with Rta in a diffuse (A: ii, B: ii) or globular pattern (A: iii, B: iii) were identified. Scale bar = 10 μm. (C) The percentages of Rta and pATM-positive 2089 or ΔG4ΔG5 cells or EA-D and p53BP-positive 2089 cells where Rta or EA-D were distributed in diffuse or globular intra-nuclear patterns were determined and the results represented as average percentages (C) (n = 2, NS = not significant, ** denotes P = 0.00015).