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. 2015 May 7;10(5):e0126088. doi: 10.1371/journal.pone.0126088

Fig 7. Induction of γH2AX is not sufficient for activation of the EBV lytic cycle in Burkitt lymphoma cell lines.

Fig 7

(A) Cell lysates of Raji cells treated with AZA, TPA, sodium butyrate (NaB), NaB and TPA, or untreated (UN) were analyzed by immunoblots with antibodies against γH2AX, EA-D, and GAPDH (A: i) or H2AX and GAPDH (A: ii); fold-stimulation values of γH2AX normalized to H2AX are indicated. (B) HH514-16 cells were irradiated with 0 Gy or 20 Gy dose of ionizing radiation (IR) and harvested after 0.5 h or 24 h, or treated with AZA for 24 h. Cell lysates were analyzed by immunoblots with antibodies against γH2AX (S135), ZEBRA, and GAPDH (B: i) or GAPDH and H2AX (B: ii); fold-stimulation values of γH2AX normalized to H2AX are indicated. (C) HH514-16 cells were irradiated with 0 Gy, 2Gy, 5 Gy, 10 Gy, 20 Gy, or treated with AZA. Total RNA was extracted from cell lysates after 24 h or 48 h and relative levels of BZLF1 mRNAs were measured by qRT-PCR using the standard-curve method. Individual samples were assayed in triplicate; in two biological replicates.