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. 2015 May 7;10(5):e0126088. doi: 10.1371/journal.pone.0126088

Fig 10. pATM is induced in response to expression of WT ZEBRA, and ZEBRA mutants Z(S186A), and Z(N182E) in 293 cells.

Fig 10

(A) 293 cells co-transfected with an empty vector (CMV) and a membrane-targeted EGFP-farnesylated construct (mGFP) (A: i) or singly transfected with expression vectors for ZEBRA (A: ii), Z(S186A) (A: iii), Z(N182E) (A: iv), Z(S186E) (A: v), or Z(R183E) (A: vi) were fixed and double-stained for ZEBRA and pATM (S1981); Arrows indicate transfected cells expressing transfected genes or pATM or transfected genes and pATM. Detector settings were kept constant during confocal image acquisition. (B) 293 cells were fixed and stained for pATM (S1981) after co-transfection with a membrane-targeted EGFP-farnesylated construct (mGFP) and an empty vector (CMV), or expression vectors for ZEBRA, or Z(S186A), or Z(N182E), or Z(S186E), or Z(R183E). The percentage of total cells that were positive for both pATM and mGFP was determined in blinded experiments (n = 4). **P<0.01; P = 0.0001 for WTZ vs. CMV, 0.00017 for Z(S186A) vs. CMV, and 0.0013 for Z(N182E) versus CMV.