Skip to main content
. 2015 May 7;10(5):e0121648. doi: 10.1371/journal.pone.0121648

Fig 5. ALDH1B1 modulates Wnt/β-catenin, Notch and PI3K/Akt signaling pathways.

Fig 5

(A) For the Wnt/β-catenin reporter assay, scramble or ALDH1B1 shRNA transfected SW480 cells were transiently transfected with either TOPflash, or FOPflash reporter plasmid along with pRL-TK vector as an internal control. Data are expressed as TOP/FOPflash ratio and presented as mean ± SEM (representative from three individual experiments done in triplicate). * P < 0.05, Student’s unpaired t-test. (B) RT-PCR analysis of RNA isolated from SW480 cells transfected with scramble shRNA or ALDH1B1 shRNA. mRNA levels were expressed as a ratio of the levels in scramble shRNA-transfected cells. GAPDH gene expression was used to normalize data. Results are presented as the mean ± SEM from three experiments. * P < 0.05, Student’s unpaired t-test, compared with results in scramble shRNA cells. Western blot analysis of expression of members of Wnt/β-catenin and Notch pathways (C) and of FABP5, PPARβ/δ and PI3K/Akt pathway members (D) were conducted in ALDH1B1 shRNA and scramble shRNA cells. Western blots were quantified by densitometry, numbers represent mean ± SEM of three independent experiments. * P < 0.05, compared to results in scramble shRNA cells.