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. 2015 May 7;10(5):e0125343. doi: 10.1371/journal.pone.0125343

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© 2015 Kuo et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 1 — (A) Western blot analysis was performed to investigate the expression of JARID1B and α-MYCN in MYCN amplification (MNA⁺) and non-MYCN amplification (MNA^-) NB cells. (B) The SP percentage was analyzed in MNA⁺ and MNA^- NB cells by Hoechst staining and flow cytometry. SK-N-BE(2) and SK-N-AS cells had 9.05% and 1.45% SP cells (C) Representative Aldeflour assay result of MNA⁺ SK-N-BE(2) and MNA^- SK-N-AS NB cells showed 17.2% and 3.43% ALDH+ subpopulation, respectively. Data was collected from three independent experiments.