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. 2015 Mar 4;168(1):175–191. doi: 10.1104/pp.114.254599

Figure 4.

Figure 4.

Anthocyanin production and qRT-PCR analysis of expression profile analysis in the flowers in transient assay of overexpression of GUS (A), PeMYB2 (B), PeMYB11 (C), PeMYB12 (D), and PebHLH1 (E) with or without the addition of PebHLH1 (F–H) in P. aphrodite ssp. formosana by A. tumefaciens infiltration. I to R, Anthocyanin contents and expression profiles in flowers with overexpression of GUS, PebHLH1 (bH1), PeMYB2 (M2), PeMYB2 + PebHLH1 (M2 + bH1), PeMYB11 (M11), PeMYB11 + PebHLH1 (M11 + bH1), PeMYB12 (M12), and PeMYB12 + PebHLH1 (M12 + bH1). The anthocyanin content and expression level detected in the negative control of overexpressed GUS were set to 1×, and those in the other overexpression flowers were calculated as fold change to those of the negative control as shown above each bar. This result was for three technological repeats, and the experiment was performed three times independently. FW, Fresh weight. Bars = 0.5 cm.