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. 2015 Mar 27;168(1):258–272. doi: 10.1104/pp.114.254672

Figure 4.

Figure 4.

Organ and tissue location of MtNramp1 transcripts. A, MtNramp1 expression in shoots, roots, and nodules of unnodulated nitrogen-fertilized and nodulated plants. MtUbiquitin carboxyl-terminal hydrolase1 (MtUb1) was used as positive control for RT-PCR. B, Histochemical staining of GUS activity in the root and nodules of M. truncatula plants transformed with plasmid pBI101 containing the MtNramp1-promoter::gus fusion. C, Longitudinal section of a GUS-stained nodule from M. truncatula plants transformed with plasmid pBI101 containing the MtNramp1-promoter::gus fusion. D, Cross section of a GUS-stained root from M. truncatula plants transformed with plasmid pBI101 containing the MtNramp1-promoter::gus fusion. Bars = 1 mm (B) and 0.1 mm (C and D).