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MtNramp1 Tnt1 insertional knockout line. A, Position of the Tnt1 insertion site for nramp1-1 (NF20333). B, RT-PCR of MtNramp1 expression in 28-dpi nodules in control (wild type [WT]) and nramp1-1 M. truncatula lines. Expression of MtUbiquitin carboxyl-terminal hydrolase (MtUb1) was used as positive control.
