| 5MtNramp1-XbaI |
AATCTAGAATGGCACATCAAGAAGTGAAT |
Cloning of MtNramp1
cDNA in pYGPE15 |
| 3MtNramp1-BamHI |
TTGGATCCTCACAACCTAGGTCCCTCTG |
Cloning of MtNramp1
cDNA in pYGPE15 |
| 5MtNramp1p-HindIII |
GGAAGCTTTTTTTATTTGAACTTAGAATTA |
Cloning of MtNramp1 promoter in pBI101 |
| 3MtNramp1p-BamHI |
TTGGATCCGTTTACTATTACTATTCACTC |
Cloning of MtNramp1 promoter in pBI101 |
| 5GWMtNramp1p |
GGGGACAAGTTTGTACAAAAAAGCAGGCTATTGAACTTAGAATTAAACCAC |
Cloning of MtNramp1 promoter and coding sequence in Gateway vectors |
| 3GWMtNramp1 |
GGGGACCACTTTGTACAAGAAAGCTGGGTACAACCTAGGTCCCTCTGGTCG |
Cloning of MtNramp1 promoter and coding sequence in Gateway vectors/C-terminal GFP fusion |
| 5MtUBqF |
GAACTTGTTGCATGGGTCTTGA |
Quantitative reverse transcription (q)PCR of MtUbiquitin carboxyl-terminal hydrolase
|
| 3MtUBqR |
CATTAAGTTTGACAAAGAGAAAGAGACAGA |
qPCR of MtUbiquitin carboxyl-terminal hydrolase
|
| 5MtNramp1 + 1GW |
GGGGACAAGTTTGTACAAAAAAGCAGGCTTCATGGCACATCAAGAAGTGAAT |
C-terminal GFP fusion of MtNramp1 |
| 5MtNRAMP1-1533 |
AAGGGGTTTGTAAACGATGGTCAGT |
qPCR of MtNramp1
|
| 3MtNRAMP1-1659 |
GCTCTCTAAAATGACTTGAAAGCAA |
qPCR of MtNramp1
|
| 5MtNRAMP2-1500 |
AACCAAGTGGAAAAAGGAATTGTGG |
qPCR of MtNramp2
|
| 3MtNRAMP2-1617 |
TTGGATCCTTTATTCTGGTAGTGGT |
qPCR of MtNramp2
|
| 5MtNRAMP3-1431 |
TTTTCAGGCATGGCAGTATATTTGG |
qPCR of MtNramp3
|
| 3MtNRAMP3-1598 |
TGGACTACTTCTCTGAGGCAATTGC |
qPCR of MtNramp3
|
| 5MtNRAMP4-1427 |
CTTCCTTTCTGAGGTGAACAGCGTA |
qPCR of MtNramp4
|
| 3MtNRAMP4-1586 |
TCCATATTTTTAATGTTGTGTAACTTGTGT |
qPCR of MtNramp4
|
| 5MtNRAMP5-1387 |
TTTCCTCTGAAGTGAGTGGAGCAGT |
qPCR of MtNramp5
|
| 3MtNRAMP5-1565 |
AACTTTGCGTGGTAGCTACTATTTG |
qPCR of MtNramp5
|
| 5MtNRAMP6-1527 |
TGTGCAACAGCATGGATTTCATTTA |
qPCR of MtNramp6
|
| 3MtNRAMP6-1663 |
CTATATATTCACATCATCATCACCAAAAA |
qPCR of MtNramp6
|
| 5MtNRAMP7-1423 |
AATAACCGCTGCATATGTTGCATTC |
qPCR of MtNramp7
|
| 3MtNRAMP7-1549 |
CTGCAGTTCTAAGGATCCATTATTCA |
qPCR of MtNramp7
|
| 5MtFRO1qF |
GGTGACACGTGGATCATCTG |
qPCR of MtFRO1
|
| 3MtFRO1qR |
TTGCAATCCACAGGAACAAA |
qPCR of MtFRO1
|
