Table I. Lignin analyses for the wild type and BdLAC5 (Bd4442) and BdLAC6 (Bd5024) misregulated mutants.
Lignin content and structure for extractive-free mature stems were measured by Klason and thioacidolysis methods. Values are means ± sd from individually analyzed plants (n = 3).
| Culture and Genotype | Lignin Content | Relative Frequency of Lignin-Derived Thioacidolysis Monomers |
||
|---|---|---|---|---|
| H | G | S | ||
| % | ||||
| First culture | ||||
| Wild type | 17.63 ± 0.01 | 2.8 ± 0.1 | 30.9 ± 0.8 | 66.3 ± 0.9 |
| Bd4442 | 15.71 ± 0.04a | 2.6 ± 0.2 | 25.3 ± 0.4a | 72.2 ± 0.6a |
| Bd5024 | 17.53 ± 0.14 | 2.8 ± 0.1 | 33.3 ± 0.3 | 63.9 ± 0.3 |
| Second culture | ||||
| Wild type | 16.93 ± 0.57 | 3.0 ± 0.2 | 30.1 ± 2.5 | 66.9 ± 2.7 |
| Bd4442 | 15.60 ± 0.07a | 1.6 ± 0.2a | 22.2 ± 3.5a | 76.3 ± 3.5a |
| Third culture | ||||
| Wild type | 17.59 ± 0.49 | 3.3 ± 0.1 | 31.0 ± 1.0 | 65.7 ± 1.0 |
| Bd4442 | 16.19 ± 0.20a | 2.7 ± 0.1 | 26.5 ± 0.5a | 70.8 ± 0.5a |
a
Significantly different from the corresponding wild-type sample (one-way ANOVA, Tukey’s honestly significant difference) at P < 0.05.