Figure 7.

OR biogenesis: link to the nucleus movements and the cell cycle. A, Position of the nuclei in the trichomes before and after the OR formation. Percentage of nuclei located in the lower part of the stalk (black), the middle of the stalk (gray), and the upper part of the stalk (white). Different letters above bars mean significant differences (χ² test; P < 0.0005); more than 100 trichomes per sample from more than 10 plants were used. Similar results were obtained from three biological replicas. B, Scheme displaying how nucleus position was categorized into three groups. C, Examples of the nucleus (stained by 4′,6-diamino-phenylindole) positions evaluated in A. D, Band of microtubules at the base, which can be spotted before OR biogenesis. E, sim mutant ectopic cell divisions (yellow arrow) do not take place at the OR (blue arrow). Bars = 20 µm.