Figure 4.

SRS images and scanning electron micrographs of Arabidopsis cuticle. The surface of Arabidopsis stems were imaged using SRS microscopy (A, C, and D) and SEM (B, D, and E). A and B, The surface of the untreated stem of wild-type Arabidopsis, ecotype Landsberg erecta, with the structure of cuticle wax crystals clearly visible. C and D, The surface of the wild-type Arabidopsis stem following a wash in hexane that dissolved the cuticle waxes. E and F, The surface of the stem from a cer1 Arabidopsis mutant. A, C, and E were generated from three-dimensional stacks taken of a 64- × 64-µm field of view and are displayed in false color.