Figure 6.

SRS spectral images of D. anthonyi cuticle. SRS spectral imaging of D. anthonyi cuticle waxes shows changes in chemical composition with depth. A and B, The SRS and SEM images, respectively, of D. anthonyi leaf showing the crystalline structure of cuticle wax deposits on the adaxial surface. The image in A is a three-dimensional reconstruction of an image stack taken from a 126- × 126-µm field of view at the 2,840 cm^–1 Raman shift. C, SRS spectral scans taken from the more superficial and deeper wax areas indicated on D. Blue rectangle, An area of superficial wax; red line, an area deeper in the cuticle. The imagess on the right and bottom of the main image in D are from the orthogonal views through the stack showing the depth profile image of the cuticle wax.