Figure 7.

Age-dependent degradation of bulk proteins is disrupted in the Osatg7-1 mutant. Soluble (A and C) and insoluble (B and D) proteins were extracted from whole leaves (A and B) and the middle, living parts (10 cm of the middle section of leaves; C and D) at different positions on the plant. Proteins from equal leaf area were applied to all lanes in the same gel for analysis by SDS-PAGE followed by staining with Coomassie Brilliant Blue R-250. The asterisk indicates accumulation of smears of small polypeptides in lower leaves of Osatg7-1. Major leaf polypeptides in the soluble fraction (LSU and small subunit [SSU] of Rubisco) and insoluble fraction (LHCII) are indicated with black arrowheads. Sizes of molecular mass (M.M.; kilodaltons) markers are indicated on the left of the stained gel.