Figure 3.

CSEP0105 and CSEP0162 interact with the barley Hsp16.9 and Hsp17.5 proteins in a Y2H assay. Yeast was transformed with bait and prey constructs. Interactions were selected on dropout (DO) medium lacking Leu (L), His (H), adenine (A), and Trp (W), supplemented with 5 mm 3-AT. The β-galactosidase assay (5-bromo-4-chloro-3-indolyl-β-d-galactopyranoside [X-gal]) was performed on a filter paper print of DO-Leu-Trp-grown yeast. SNF4/SNF1 interaction was used as a positive control, and CSEP0081 and CSEP0254 were used as negative controls.