Figure 7.

Hsp16.9 protects the heat denaturation of E. coli proteins in vitro. Thermostability is shown for total protein from E. coli expressing GUS or Hsp16.9. Cell-free total protein extracts (250 µg mL⁻¹) were heated for 15 min. Heat-denatured proteins were removed by centrifugation, and the protein content of the supernatant fractions was determined. Values are relative to the unheated controls. Data shown are means of three replicates ± se. Asterisks show significant differences (P < 0.05) between the GUS control and Hsp16.9 at each heat treatment analyzed by Duncan’s multiple range test. The experiment was repeated once with a similar result.