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. 2015 Mar 13;168(1):321–333. doi: 10.1104/pp.15.00278

Figure 9.

Figure 9.

Localization of CSEP0105, CSEP0162, Hsp16.9, and Hsp17.5 proteins in Bgh-infected barley cells. Confocal images show cells transiently cotransformed by particle bombardment with the respective constructs as described in Figure 5. The Hsp16.9-mCherry and Hsp17.5-mCherry fluorescent signals are in the cytosol (C). YFP-CSEP0105 fluorescent signal is in the cytosol when coexpressed with Hsp16.9-mCherry and in the cytosol and nucleus (N) when coexpressed with the free mCherry construct. YFP-CSEP0162, as well as the free YFP and mCherry marker proteins, localized to the cytosol, nucleus, and EHMx. The fluorescent signals labeled EHMx also include the cytosol surrounding the haustoria (H). Since the nucleus and haustoria were not found in a single confocal plane, images showing nuclei of the same cells are presented in Supplemental Figure S3. Bars = 20 µm.