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. 2015 Mar 31;168(1):94–106. doi: 10.1104/pp.15.00315

Figure 4.

Figure 4.

Conversion of (+)-sabinene (1) by CYP750B1 and CYP76AA25 producing stereoselectively (+)-trans-sabinol (2). Shown are total ion chromatograms of (+)-sabinene conversion by CYP750B1 (A), (+)-sabinene conversion by CYP76AA25 (B), empty vector control reactions (C), and authentic standards (D). Peaks correspond to (+)-sabinene (1), (+)-trans-sabinol (2), (+)-sabinone (3), and (+)-cis-sabinol (4). Insets show the mass fragmentation pattern of CYP750B1 assay product peak 2 (E) and CYP76AA25 assay product peak 2 (F) coeluting with the (+)-trans-sabinol authentic standard (G). Enzyme and control reactions were incubated for 1 h at 30°C. Pentane extracts of assays and standards were separated on a DB1 column. Rt, Retention time.