Farnesene conversion by CYP76AA25. Shown are total ion chromatograms of conversion of farnesene (1) in assays with CYP76AA25 (A) and lack of conversion of farnesene in negative control assays without CYP76AA25 after 1 h of incubation at 30°C and separation of the pentane extract on a DB1 column (B). The reaction product (2) formed could not be identified with any of our available authentic standards. Based on mass fragmentation pattern (C), this product is similar or identical to 2,6,10-trimethyldodeca-2,6,10-trienal. Rt, Retention time.