Figure 9.

NADH-dependent conversion of (+)-trans-sabinol but not (+)-cis-sabinol by cell-free protein extracts of WRC tissue. (+)-trans-Sabinol is converted to (+)-sabinone by cell-free protein extracts. Assays containing soluble protein extracts were incubated for 90 min at 30°C with either (+)-trans-sabinol or (+)-cis-sabinol with and without NADH. Assays products were extracted, separated on a DB1 column, and analyzed by GC-MS. Sabinone production was confirmed by comparing product peak retention time and mass fragmentation with authentic standards, and areas were calculated for ion 108 (mass to charge ratio). Shown are average values for quadruplicate experiments. *, Significant differences with and without NADH added in assays with (+)-trans-sabinol as determined by t test (P < 0.05).