Table II. Relative catalytic activity of CYP76AA25 and CYP750B1 with different monoterpene, sesquiterpene, and nonterpenoid substrates.
| Substrate | CYP76AA25 kcat | CYP750B1 kcat |
|---|---|---|
| % | ||
| Monoterpenes | ||
| (+)-Sabinene | 100a | 100b |
| (R)-Limonene | 0 | 0 |
| (S)-Limonene | ndc | ndc |
| 3-Carene | 0 | 0 |
| Geraniol | 0 | 0 |
| Linalool | 0 | 0 |
| Nerol | ndc | 0 |
| Terpinolene | 0 | 0 |
| Sesquiterpenes | ||
| Farnesene | 29 | 0 |
| Cadinene | 0 | 0 |
| Caryophyllene | 0 | 0 |
| Cedrene | 0 | 0 |
| Germacrene | 0 | 0 |
| Humulene | 0 | 0 |
| Longifolene | 0 | 0 |
| Nonterpenoid | ||
| Naringenine | 0 | 0 |
| Isoproturon | 5 | 0 |
| 7-Methoxycoumarine | 0 | 0 |
| Matairesinol | 0 | 0 |
a
100% is 1.30 min−1.
b
100% is 0.20 min−1.
c
Trace activity of <0.1% substrate conversion in standard assays (kcat) could not be determined.