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. Author manuscript; available in PMC: 2016 Jun 1.
Published in final edited form as: Immunobiology. 2014 Dec 30;220(6):823–832. doi: 10.1016/j.imbio.2014.12.011

Figure 2. TLR3 was associated with SREC-I in the presence of Poly I:C in HEK293 cells.

Figure 2

HEK293 cells stably expressing TLR3 was transfected with SREC-I-GFP for 22 hr. Cells were then treated with or without 10ug/ml Poly I:C for 30 min. Cell lysates were collected and SREC-I-GFP was immunoprecipitated using anti-GFP antibody and anti GFP ab and then the precipitated complexes were subjected to SDS-PAGE analysis followed by blotting for TLR3 using anti-TLR3 antibody. Experiments were carried out twice, reproducibly.