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. Author manuscript; available in PMC: 2016 May 15.
Published in final edited form as: Dev Biol. 2015 Mar 19;401(2):299–309. doi: 10.1016/j.ydbio.2015.02.026

Figure 6. Knockdown of sing in both FCs and FCMs results in lethality and reduction in muscle nuclei numbers.

Figure 6

(A) Horizontal sections of 13h APF animal to show activities of gal4 drivers used in FCs (arrows) and FCCs. UAS-lacZ was crossed to each driver, and samples were stained with anti-β-galactosidase (green) and DAPI (blue) to visualize nuclei. Mef2-gal4 is active in all myoblasts; rp298-gal4 is active in FCs; and sns-gal4 is active in FCMs close to the template. βGal accumulation in founder cell nuclei of sns>lacZ samples probably arises from fusion of βGal-positive FCMs to the template. (B–E) Horizontal sections of pharate adults stained for accumulation of F-actin (Phalloidin) and MEF2. (B’–E’) Higher magnification views of muscle fibers and MEF2-positive nuclei (B–B’) Wild type; (C–C’) sing knockdown in founder cells; (D–D’) sing knockdown in fusion competent myoblasts. (E–E’) sing knockdown in founder cells plus fusion competent myoblasts. Note that muscle formation appears normal in all genotypes, but the size and number of nuclei is reduced in E and E’. (F) Quantification of average nuclei counts per unit area. Samples from the double driver are the only group that shows a significant reduction in nuclei number (*p>0.05). Scale bar, 20 µm.