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. 2015 Apr 28;5:13. doi: 10.1186/s13395-015-0040-z

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© McCourt et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Spectra and melt curves obtained by circular dichroism spectroscopy. (A-C) Circular dichroism absorption spectra from 200 to 260 nm for exon-skipped dystrophins (A), gene therapy constructs (B), and utrophins (C). Molar ellipticity [θ], with units of degrees centimeter squared per decimole, was calculated as θ / (10 × c × l) where c is the molar concentration of the sample (mole/L) and l is the path length in cm. (D-F) CD absorption spectra monitored at 222 nm from 20°C to 90°C for exon-skipped dystrophins (D), gene therapy dystrophins (E), and utrophins (F). Melt curves were normalized to θ₂₂₂ from 0 to 1 fraction unfolded and a representative curve plotted. See Table 1 for melting temperatures (CD T_m1 and T_m2). hDys - human dystrophin, hUtr - human utrophin.