. Author manuscript; available in PMC: 2017 Feb 1.

Published in final edited form as: Eur J Drug Metab Pharmacokinet. 2014 Nov 7;41(1):55–67. doi: 10.1007/s13318-014-0233-6

Table 1. A comparison of the LC-MS/MS analytical methods for mouse and dog plasma.

Parameter	Method for Mouse Plasma	Method for Dog Plasma
Sample Volume	100 μL	150 μL
Sample Preparation	Methanol protein precipitation	MTBE liquid/liquid extraction under basic conditions
MS Instrumentation	AB Sciex API 3000	AB Sciex 5500 QTrap^®
Injection Volume	20 μL	20 μL
Calibration Range	0.250 – 100 ng/mL	2.50 – 2,500 pg/mL
Lower Limit of Quantitation	250 pg/mL	2.50 pg/mL
Run Time	9.0 min	12 min