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Coverage of the PIK3CA gene sequencing using the AmpliSeqCancer Panel (v1) kit for 8 FFPE OPSCC tumor specimens. a Aligned (hg19) reads of 4 HPV(+) and 4 HPV(−) tumor specimens are shown in relation to the PIK3CA locus and AmpliSeq Cancer (versions 1 and 2) and TruSight tumor library designs (below reads). b Coverage of exon 9 of the PIK3CA gene using the AmpliSeq (v1) kit shows the c.1633G>A (p.E545K) mutation in the HPV−_1 sample
