FIGURE 1.
Initial characterization of HypE-specific VHHs by immunoblotting and overview of the generation of VHHs. A, schematic representation of VHH generation. B, an initial test of 10 putative HypE-specific VHHs in immunoblot assays. His6-VHHs were probed against GST-HypEΔ83 and detected using an anti-His6-HRP antibody. C, evaluation of cross-reactivity of VHHs binding to GST-HypEΔ83. His6-tagged VHHs were probed against GST-HypEΔ83 and GST-VopS and detected using an anti-His6-HRP antibody. D, sequence alignment of characterized anti-HypE VHHs, including an anti-GFP VHH (enhancer) as reference. CDR1–CDR3 are underlined.