FIGURE 2.

RAG2 core and Mini-RAG1 are monomers. A, Western blot analysis using anti-RAG2 antibody of fractions from size-exclusion chromatography of whole cell extracts containing GST-R2c (upper) or MBP-R2c (lower), with lanes aligned with the fraction from which they are derived. At the top is shown the elution profile of molecular mass standards. B, size-exclusion chromatography profiles of purified Mini-RAG1 (blue), MBP-tagged Mini-RAG1 (red), and molecular mass standards (black). C, sensorgrams obtained using biosensors loaded with MBP-R1c, incubated with 3.8 μm MBP-R2C or GST-R2C. D, schematic diagram of RAG protein combinations analyzed by biolayer interferometry. mAU, milliabsorbance units at 280 nm.