FIGURE 4.
Quantitation of the RAG1-RAG2 interaction. A, diagram of RAG1 proteins used in affinity measurements. B–D, sensorgrams obtained using GST-R2c-loaded biosensors, incubated with the indicated concentrations of MBP-R1c, MBP-Mini-RAG1, and Mini-RAG1, respectively. E, sensorgrams were fit globally to a 1:1 binding model, and the equilibrium dissociation constant (KD) and kinetic constants (ka and kd) were calculated. F, KD values obtained in experiments with MBP-Mini-RAG1 loaded biosensors and MBP-R2c in solution. The results of three independent measurements are shown with the average in parentheses.