FIGURE 5.

Expression of piRNA-associated protein Mili is increased in testes of Mtdh^−/− mice. A, expression of Miwi, Mili, and Rad51 in testes lysates from WT (+/+), heterozygous (+/−), and homozygous Mtdh knock-out mice (−/−) by Western blotting. B, Mili expression in testes from WT and Mtdh^−/− mice by immunofluorescent staining. Green, Mili; blue, nuclei (DAPI). C, Miwi expression in testes from WT and Mtdh^−/− mice by immunofluorescent staining. Arrows indicate nuclei of sperm in WT testes, which are devoid of Miwi staining. Green, Miwi; blue, nuclei (DAPI), blue.