FIGURE 2.

Tadalafil inhibits high glucose-stimulated events in mRNA translation. Podocytes were incubated with 5 or 30 mm glucose (GLU) for 5 (A–C) and 30 min (D) with or without preincubation with 10 μm tadalafil (TAD) for 8 h. Cell lysate protein was immunoblotted with specific antibodies. A, antibody against 4E-BP1 phosphorylated on Thr-36/47 (P-4E-BP1) or 4E-BP1. B, antibody against p70S6 kinase phosphorylated on Thr-389 (P-S6K) or p70S6 kinase (S6K). C, antibody against eEF2 kinase phosphorylated on Ser-366 (P-eEF2K) and eEF2 kinase (eEF2K). D, antibody against eEF2 phosphorylated on Thr-56 (P-eEF2) and eEF2. In A–D, composite data from three to four experiments are shown in histograms; error bars represent S.E.