Skip to main content
. 2015 Mar 20;290(19):12079–12089. doi: 10.1074/jbc.M114.624999

FIGURE 2.

FIGURE 2.

Characterization and erythroblast differentiation of corrected β-Thal iPS cells. A, flow cytometry expression analysis of iPS cell-specific markers in βThal654_iPSCre16 cells. Red, isotype control; blue, antigen staining for OCT4 or SSEA4. B, quantitative reverse transcription-PCR analysis of endogenous OCT4, SOX2, and NANOG expression in βThal654_iPS, βThal654_iPSCre16, and H1 (human embryonic stem cell line) as a positive control. The data are presented as mean ± S.D. (error bars) from three assays. C, H&E staining of teratomas derived from βThal654_iPSCre16 cells. Scale bars, 200 μm. D, bright field images of cfu derived from βThal654_iPSCre16 cells. G, granulocytes; E, erythroblasts; M, megakaryocytes. E, ratio of different types of colonies counted at day 21 after differentiation. E, erythroblasts; G, granulocytes; M, megakaryocytes; GM, granulocytes and megakaryocytes; GEMM, four types of colonies, including granulocytes, erythrocytes, megakaryocytes, and macrophages. F, flow cytometry expression analysis of HBB in erythroblasts derived from βThal654_ iPS and βThal654_iPSCre16 cells. Black, isotype control; orange, antigen staining for HBB.