TABLE 1.
Select genes identified in EHD3−/− screen
Select down-regulated genes.
| Gene name | Protein | Function | -Fold increase/decrease (vs WT) | p value |
|---|---|---|---|---|
| Ehd3 | EHD3 (negative control) | Regulator of endosomal transport | −3.6359 | 0.0001 |
| Sln | Sarcolipin | SERCA regulating protein. Dominantly expressed in atria, with smaller ventricular expression | −1.9642 | 0.016 |
| Slc8a1 | Na/Ca exchanger | Promotes proper Ca homeostasis and EC coupling | −1.6789 | 0.0031 |
| Cacna1h | T-type Ca channel, α1H subunit | (Also known as CaV3.2) Intimately involved in pacemaker activity of SAN cells, small distribution in ventricle | −1.6789 | 0.047 |
| Strn | Striatin | Involved in vesicular trafficking and signal transduction | −1.483 | 0.0009 |
| Vit | Vitrin | Part of the Willebrand A domain, involved in cell adhesion | −1.4811 | 0.0051 |
| Stard10 | StarD | StarD family is involved in protein-related lipid transfer and lipid transport systems | −1.4192 | 0.0335 |
| Trdn | Triadin | Regulating RyR2 response to SR luminal Ca | −1.4098 | 0.086 |
| Scn4b | β1 subunit of voltage-gated NaV1.4 | Excitability of myocyte; slows inactivation and hastens recovery. Mutations linked to LQTS 3 | −1.3139 | 0.067 |
| Scn10a | Voltage-gated NaV1.8 | Regulation of myocyte excitability. Loss of function mutants associated with heart block and ventricular arrhythmia | −1.2786 | 0.0213 |
| Foxi1 | Forkhead box I1 | Regulation of H+-ATPase (proton pump) | −1.2813 | 0.011 |
| Taar7b | Trace amine-associated receptor, 7B | G-protein coupled chemosensory receptor, little known regarding function in heart | −1.2705 | 0.0125 |
| Rcan1 | Rcan | Regulator of calcineurin | −1.2634 | 0.0409 |
| Snx10 | Sorting nexin 10 | Involved in endosomal sorting complexes, localized to early endosomes | −1.2581 | 0.0034 |
| Atp4b | H+/K+ pump | Acidification of early endosomes after internalization | −1.2243 | 0.0040 |
| Ak4 | Adenylate kinase 4 | Governs metabolic response, AMP-dependent signaling, localized to mitochondrial matrix | −1.2158 | 0.0071 |
| Rep15 | RAB15 effector protein | Involved in protein recycling out of the endosomal recycling compartment | −1.1973 | 0.0227 |
| Myom1 | Myomesin 1 | Sarcomeric protein; known to be upregulated in dilated myopathies | −1.197 | 0.0015 |
| Rab39 | RAB39 | Member of the Ras oncogene family, linked to endosomal trafficking | −1.195 | 0.05 |
| Slc26a3 | Chloride/sulfate transporter | Ion homeostasis | 1.7762 | 0.0011 |
| Ide | Insulin degrading enzyme | Metabolic response, response to natriuretic peptide-mediated signaling, etc. | 1.509 | 0.0002 |
| Kcne1 | Voltage-gated K+ channel β subunit, Isk-related subfamily | Beta subunit of the slow, voltage gated K channel. Mutations linked to torsade de pointes | 1.397 | 0.0241 |
| Pcdhb2 | Protocadherin β2 | Cadherin family protein, structural & cell adhesion | 1.3568 | 0.024 |
| Cldn10 | Claudin 10 | Component of tight junctions | 1.3396 | 0.0165 |
| Kcnj8 | Inward-rectifying K channel | K(ATP) channel, membrane excitability | 1.2272 | 0.0253 |
| Adrb2 | β2-AR | Mediates response to catecholamines | 1.2092 | 0.044 |
| Snx39 | Sorting nexin 29 | Involved in endosomal sorting complexes | 1.2066 | 0.0279 |
| Atp2a3 | SR/ER Ca ATPase3 (SERCA3) | Regulated SR Ca uptake in heart, minor component | 1.1804 | 0.0341 |
| Calb1 | Calbindin 1 | Ca binding protein, mediates Ca absorption, heart function unclear | 1.1482 | 0.0178 |
| Rab11fip2 | RAB11 interacting protein 2 | Known to interact with EHD3 in early endosomal transport | 1.1312 | 0.01 |
| Scnn1b | Beta unit of the nonvoltage-gated Na channel | Maintains proper epithelial tension, epithelial distribution | 1.1065 | 0.0136 |