TABLE 1.
Bacterial strains and plasmids used in this study
ApR, KmR, NalR, and TcR indicate resistance to ampicillin, kanamycin, nalidixic acid, and tetracycline, respectively.
| Strain | Relevant characteristics | Reference |
|---|---|---|
| E. coli | ||
| DH5α | supE44 Δ (lacZYA-argF)U169 φ80 lacZDM15 hsdR17 (rK− mK+) recA1 endA1 gyrA96 thi1 relA1; NalR | Ref. 53 |
| TOP10F′ | F′[lacIq, Tn10 (TetR)] mcrA Δ(mrr-hsdRMS-mcrBC) Φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(ara leu) 7697 galU galK rpsL (StrR) endA1 nupG; TcR | Invitrogen |
| P. aeruginosa | ||
| PAO1 pvdF ΔfoxR | PAO1 pvdF::KmR with a deletion of amino acids 12–295 of the FoxR (PA2467) protein, KmR | Ref. 25 |
| Plasmid | ||
| pMMB67EH | IncQ broad host range plasmid, lacIq; ApR | Ref. 27 |
| pMMB/HA-FoxR | pMMB67EH carrying the P. aeruginosa foxR gene (PA2467), which has been N-terminally HA-tagged; ApR | Ref. 22 |
| pMMB/HA-FoxR-S166A | pMMB/HA-FoxR in which serine 166 has been mutated to an alanine; ApR | This study |
| pMMB/HA-FoxR-D169A | pMMB/HA-FoxR in which aspartic acid 169 has been mutated to an alanine; ApR | This study |
| pMMB/HA-FoxR-S172A | pMMB/HA-FoxR in which serine 172 has been mutated to an alanine; ApR | This study |
| pMMB/HA-FoxR-H175A | pMMB/HA-FoxR in which histidine 175 has been mutated to an alanine; ApR | This study |
| pMMB/HA-FoxR-A189G/L190G | pMMB/HA-FoxR in which alanine 189 and leucine 190 have both been mutated to glycine; ApR | This study |
| pMMB/HA-FoxR-T192S | pMMB/HA-FoxR in which threonine-192 has been mutated to a serine; ApR | This study |
| pMMB/HA-FoxR-T192C | pMMB/HA-FoxR in which threonine 192 has been mutated to a cysteine; ApR | This study |
| pMMB/HA-FoxR-T192V | pMMB/HA-FoxR in which threonine 192 has been mutated to a valine; ApR | This study |
| pMMB/HA-FoxR-S208A | pMMB/HA-FoxR in which serine 208 has been mutated to an alanine; ApR | This study |
| pMMB/HA-FoxR-S211A | pMMB/HA-FoxR in which serine 211 has been mutated to an alanine; ApR | This study |
| pMPR8b | pMP220 carrying the P. aeruginosa foxA::lacZ transcriptional fusion; TcR | Ref. 1 |