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. 2015 Apr 1;290(19):12300–12312. doi: 10.1074/jbc.M115.644005

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FIGURE 2. — 14-3-3 proteins bind to the central domain of Exo1 and suppress its damage association. A, coimmunoprecipitation of 14-3-3s with FLAG-tagged Exo1(WT), Exo1(ΔCR), and Exo1(CR) expressed in human 293T cells. The immunoprecipitates (IP) and 5% of the input proteins are shown. IB, immunoblot. B, coimmunoprecipitation of 14-3-3s with GFP-Exo1(WT) or GFP-Exo1(ΔCR) in 293T cells expressing mCherry, mCherry-Difopein(WT), or mCherry-Difopein(MUT). C, left panels, representative images for the damage association of GFP-Exo1(WT) (top panel) or GFP-Exo1(ΔCR) (bottom panel) in cells overexpressing mCherry, mCherry-Difopein(WT), or mCherry-Difopein(MUT). Right panels, quantified results for the damage association of GFP-Exo1(WT) (top panel) or GFP-Exo1(ΔCR) (bottom panel) during the first 20 min after laser irradiation. Each data point is the average of independent measurements from five cells. Error bars represent mean ± S.D.