FIGURE 5.

The central region of Exo1 limits DNA end resection activity in a Xenopus egg extract. A, immunodepletion (depl) of the Xenopus Dna2 protein (xDna2) or of both xDna2 and xExo1 from the Xenopus NPE. B, physical association of Exo1(WT)-His and Exo1(ΔCR)-His with a bead-immobilized, 2-kb DNA fragment in xExo1-depleted NPE. C, DNA end resection activity toward a 3′ ³²P-labeled, 6-kb dsDNA fragment in the Exo1 and Dna2 codepleted NPE depicted in A that was supplemented with Exo1(WT)-His or Exo1(ΔCR)-His. Vertical lines were added for sample lane identification.