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Recombinant TprIFl and TprCFl, but not TprF, are heat-modifiable. Heat modifiability of folded recombinant proteins (A and B) and native TprI in T. pallidum (C). Following SDS-PAGE with (+) or without (−) boiling in sample buffer, proteins were stained with GelCode® Blue (A and B) or transferred to nitrocellulose (C) for immunoblotting with TprI-specific antiserum. Molecular mass standards (kDa) are on the left of each panel.
